Journal: Molecular Medicine

Article Title: Porphyromonas gingivalis promotes the formation and development of type 2 diabetes mellitus complicated with cognitive impairment by regulating gut microbiota and metabolic pathways

doi: 10.1186/s10020-025-01342-y

Figure Lengend Snippet: Effects of Pg on SCFA receptor in T2DM mice. A : The expression of SCFA receptor Olfr78 in the hippocampus was detected by immunofluorescence. Magnification: 400×. B : The mean fluorescence intensity of Olfr78. C : WB was used to detect the expression of Olfr78 in the hippocampus. D : The relative protein expression level of Olfr78. n = 6, * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: After sealing with 5% skim milk, the primary antibody Olfr78 (PA5-75357, Invitrogen, USA) and β-actin (AC026, abclonal, China) were incubated at 4°C overnight, and the secondary antibody (S0001, Affinity biosciences, China) was incubated at room temperature for 2 h. After the reaction, the color reaction was carried out by ECL (17046, zen-bio, China) and Tanon fluorescence image analysis system (5200 Multi, Tanon, China), and the relative protein expression of Olfr78 was calculated.

Techniques: Expressing, Immunofluorescence, Fluorescence